Duonix Beatrice biodiesel plant started commercial operation in 2016 with the capacity to produce 50 million gallons. Two other commercial biodiesel plants in Nebraska (Horizon Biofuels, Inc., and Northeast Nebraska Biodiesel LLC) have the capacity to produce 5.4 million gallons although both plants have ceased production.
- Duonix Beatrice, a joint venture between Flint Hills Resources and Benefuel, Inc., retrofitted the biodiesel plant in Beatrice, Nebraska, and started commercial operation in 2016. The plant is expected to produce 50 million gallons per year. The plant was built in 2007 and purchased in late 2011 by Duonix Beatrice.
- Horizon Biofuels, Inc., had been making biodiesel for use in their own equipment. They then moved from small "home brew" batches to becoming the first commercial biodiesel operation in the state of Nebraska in September 2006. They had plans to expand their presence in the market by building a 6.2-million-gallon production facility, but those plans were on hold.
- Northeast Nebraska Biodiesel LLC built a facility in Scribner with the capacity to produce five million gallons of biodiesel per year. The facility is Nebraska's only commercial-size soy biodiesel plant. Due to high soybean prices and low fuel prices, the plant closed at the end of October 2009, but with the right market conditions, there is always a possibility that the plant could restart biodiesel production.